Methylene Blue - Powder
Product Code: CH002
CAS Number
61-73-4
Colour Index Number
52015
Chemical Formula
C16H18ClN3S
Molecular Weight
319.85 g/mol
Appearance
Burgundy Powder
HS Code
3204 13 00
Methylene Blue Powder
Methylene blue is a cationic dye and antiseptic substance used in laboratories, medicine, water treatment, and fish care. In powder form, it is usually dark red (burgundy) in color, and forms a blue color when dissolved in water.
Methylene Blue Solubility Information
Methylene blue is a dye and chemical compound with the ability to dissolve in water and organic solvents. Its solubility varies depending on the type of solvent, temperature, and pH level.
General Solubility Values
| Solvent | Solubility Status |
|---|---|
| Water (H₂O) | Very soluble (~40 g/L, 25°C) |
| Ethanol (C₂H₅OH) | Moderately soluble |
| Methanol (CH₃OH) | Soluble |
| Acetone (C₃H₆O) | Low solubility |
| Chloroform (CHCl₃) | Insoluble |
| Benzene (C₆H₆) | Insoluble |
| DMSO (Dimethyl sulfoxide, C₂H₆OS) | Soluble |
| Acidic Solutions | Solubility increases |
| Basic Solutions | Solubility decreases |
Chemical Properties and Reactions
- It is a cationic (positively charged) dye.
- It has oxidative and reductive properties.
- It remains more stable in high pH (basic) environments.
- It increases oxygen-carrying capacity by donating electrons in conditions such as methemoglobinemia.
- It is used as an indicator in some oxidation-reduction reactions.
Areas of Use
Microbiology and Laboratory Use
- It is used in staining bacteria and fungi (Gram staining and Loeffler's methylene blue method).
- It is preferred to highlight cellular structures in blood smears.
- Redox Indicator: It is used to determine the redox state in chemical reactions.
- pH Indicator: It is used in laboratory tests to determine acidic and basic environments.
- DNA and RNA Staining: It is used to visualize DNA and RNA in biological samples.
Medical Use
- It increases oxygen-carrying capacity in the treatment of methemoglobinemia.
- It is used as an antiseptic (in urinary tract infections and wound cleaning).
- It is being investigated at low doses in the treatment of some psychiatric diseases.
- Fungal and Parasite Treatment: It is used in the treatment of some fungal and parasitic infections.
Forensic Medicine and Forensic Sciences
- Fingerprint Detection: It can be used in some fingerprint analyses in forensic sciences.
- Poisoning Detection: It is used in tests to determine the presence of certain toxins and chemicals.
Aquaculture and Aquarium Use
- It is used as an antifungal and antibacterial agent in the treatment of fish diseases.
- It protects eggs from fungal infections.
- It is used for water disinfection in freshwater and saltwater aquariums.
Water Treatment and Chemical Applications
- It is used in some oxidation-reduction reactions in industry.
- It serves as an indicator in water pollution tests and chemical analyses.
Methylene Blue Aggregate Test
Methylene blue test is used to determine the amount of clay, silt, and other harmful fine material in fine aggregate. It is widely applied to evaluate the quality of fine aggregate used especially in concrete and asphalt production.
Leak Test
- Pharmaceutical and Food Packaging: Leak testing of blister packs, bottles, and caps.
- Sterile Medical Devices: Testing the integrity of injectable solutions, serum bags, and sterile medical packaging.
- Waterproofing and Construction: Leak detection in waterproofing membranes, pipes, or tanks.
- Filtration Systems: Checking whether filters have any leaks.
